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Eicosanoid Protocols

  • Book
  • © 1999

Overview

Editors:
  1. Elias A. Lianos

    1. Medical College of Wisconsin, Milwaukee

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Part of the book series: Methods in Molecular Biology (MIMB, volume 120)

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About this book

In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids.
Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.



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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Use of a Retroviral Vector for Expression of a Foreign Gene

    • Brent W. Miller, Aubrey R. Morrison
    Pages 1-9

  3. Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues

    • William S. Powell
    Pages 11-24

  4. Assessment of Cyclooxygenase RNA Expression by Northern Hybridization

    • Zhonghong Guan, Aubrey R. Morrison
    Pages 25-33

  5. Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin

    • ShaAvhree Y. Buckman, Alane T. Koki, Dorothy A. Edwards, Alice P. Pentland
    Pages 35-43

  6. Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography

    • Mukut Sharma, Beatrice Hucke, Elias A. Lianos
    Pages 45-54

  7. Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry

    • M. Kerry O’Banion, John A. Olschowka
    Pages 55-66

  8. Assessment of Cyclooxygenase Protein Expression by Western Blotting

    • Zhonghong Guan, Aubrey R. Morrison
    Pages 67-76

  9. Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells

    • Alfred N. Fonteh
    Pages 77-89

  10. Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction

    • Alfred N. Fonteh, William Reed, James M. Samet
    Pages 91-105

  11. Enzyme immunoassays (EIA) of Eicosanoids

    • Kingsley Yin, Lloyd J. Forman
    Pages 107-118

  12. Transcellular Regulation of Eicosanoid Biosynthesis

    • Karsten Gronert, Clary B. Clish, Mario Romano, Charles N. Serhan
    Pages 119-144

  13. Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry

    • Samuel C. Blackman, Catherine Borg, David C. Yeomans, Guy C. Le Breton
    Pages 145-171

  14. Epoxyeicosatrienoic Acids

    • John D. Imig
    Pages 173-192

  15. Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts

    • Srinivasa T. Reddy, Harvey R. Herschman
    Pages 193-200

  16. Assessment of 5-Lipoxygenase Activity and Cellular Distribution

    • Robert A. Lepley
    Pages 201-211

  17. Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids

    • Eric G. Spokas, Joshua Rokach, Patrick Y. -K. Wong
    Pages 213-247

  18. Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition

    • Eric G. Spokas, Nancy S. Nicholson, Osman D. Suleymanov, Gerald M. Walsh, David Tuffin
    Pages 249-256

  19. Novel Eicosanoids

    • L. Jackson Roberts II, Cynthia J. Brame, Yan Chen, Jason D. Morrow
    Pages 257-285

  20. Back Matter

    Pages 287-296
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Reviews

"...well-produced, timely book..."-The Quarterly Review of Biology

"A very useful book for all specialists on eicosanoid acids, which was lacking until now in the literature." - Cellular and Molecular Biology

Editors and Affiliations

  • Medical College of Wisconsin, Milwaukee

    Elias A. Lianos

Bibliographic Information

  • Book Title: Eicosanoid Protocols

  • Editors: Elias A. Lianos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592635

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Humana Press 1999

  • Softcover ISBN: 978-0-89603-667-3Published: 25 March 1999

  • eBook ISBN: 978-1-59259-263-0Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 296

  • Topics: Biochemistry, general

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