Overview
- Editors:
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Kelly P. Kearse
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Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville
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About this book
The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology. Invariably, in putting together a book like this it is difficult to decide which methods to include and which to leave out. To this end methods were selected from a variety of disciplines, including cellular immunology, b- chemistry, and molecular biology, to try to provide something of interest for everyone who works on T-cell development and activation. I would like to mention that my primary reason for agreeing to put this book together is that, when I was a graduate student, I purchased a copy of Selected Methods in Cellular Immunology by Mishell and Shigii which proved a tremendous help in learning the basics of one-and two- dimensional gel te- niques (and other methods). The cover has long since fallen off, but it still remains one of my most valued reference books for the laboratory. It is my hope that T Cell Protocols: Development and Activation will prove similarly useful to current and future scientists wishing to learn new methods for expl- ing the development and activation of T cells.
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Table of contents (29 protocols)
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Overview: For Chapters 2 – 15
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- M. Albert. Basson, Rose Zamoyska
Pages 3-22
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T Cell Development
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- Terrence G. Gardner, Kelly P. Kearse
Pages 47-53
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- Alison M. Michie, Juan Carlos Zúñiga-Pflücker
Pages 55-62
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- Ronald Rooke, Christophe Benoist, Diane Mathis
Pages 69-79
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- Takaji Matsutani, Takeshi Yoshioka, Yuji Tsuruta, Shoji Iwagami, Tomoko Toyosaki-Maeda, Ryuji Suzuki
Pages 81-101
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- Karl J. Franek, Robert Chervenak
Pages 103-116
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- Caroline D. V. A. Bishop, Amy Jost, Elizabeth Crane, Jennifer A. Punt
Pages 117-131
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- Luminita A. Stanciu, Ratko Djukanović
Pages 133-141
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- Toshio Kitamura, Yoshihiro Morikawa
Pages 143-152
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- Melanie S. Vacchio, Elizabeth W. Shores
Pages 153-176
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- Ellen R. Richie, Dawn A. Walker
Pages 177-184
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- Janice White, John Kappler, Philippa Marrack
Pages 185-193
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Overview for Chapters 17–29
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Front Matter
Pages 195-195
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Reviews
"...The protocols themselves are highly detailed, and also refer to a notes section in each chapter which is used to impart tips and tricks which are usually essential to getting a technique to work well, but which do not often come over in the usual material and methods section of papers. Each of the chapters is also well referenced. The book would be invaluable for establishing a particular technique or set of techniques in an environment where there was little or no previous experience of such as as teaching aid for when new research staff or students come into a laboratory."- The Journal of Infection
Editors and Affiliations
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Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville
Kelly P. Kearse