Overview
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 180)
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About this book
The past decade has witnessed a spectacular explosion in both the devel- ment and use of transgenic technologies. Not only have these been used to aid our fundamental understanding of biologic mechanisms, but they have also faci- tated the development of a range of disease models that are now truly beginning to impact upon our approach to human disease. Some of the most exciting model systems relate to neurodegenerative disease and cancer, where the availability of appropriate models is at last allowing radically new therapies to be developed and tested. This latter point is of particular significance given the current concerns of the wider public over both the use of animal models and the merits of using genetically modified organisms. Arguably, advances of the greatest significance have been made using mammalian systems—driven by the advent of embryonic stem-cell–based strategies and, more recently, by cloning through nuclear transfer. For this reason, this new edition of Transgenesis Techniques focuses much more heavily on manipulation of the mammalian genome, both in the general discussions and in the provision of specific protocols.
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Table of contents (15 protocols)
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Transgenesis in Domestic Species
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Front Matter
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Characterization and Analysis of Transgenic Strains
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Front Matter
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Back Matter
Reviews
From Reviews of the First Edition
"...a must in any laboratory..." -Cellular and Molecular Biology
"...should be useful to anyone currently involved in using transgenesis as a technique..."
-Journal of Heredity
Second Edition:
"...the book remains an impressive and interesting book that will be a useful reference to molecular and developmental geneticists at all levels and potential tool for teaching graduate students and advanced undergraduates." - Human Genetics
"It will provide a valuable source of information. It will also remind you of the enormous potential that transgenesis has to offer the biologist. Transgenic research will underpin many future applications from evaluating viral vectors for gene therapy and vaccination to bioreactors for the production of proteins, from models of disease to screening tools for biological activity, from genome mapping to gene function. This book details protocols to let you participate in this." -Cloning and Stem Cells
"...a must in any laboratory..." -Cellular and Molecular Biology
"...should be useful to anyone currently involved in using transgenesis as a technique..."
-Journal of Heredity
Second Edition:
"...the book remains an impressive and interesting book that will be a useful reference to molecular and developmental geneticists at all levels and potential tool for teaching graduate students and advanced undergraduates." - Human Genetics
"It will provide a valuable source of information. It will also remind you of the enormous potential that transgenesis has to offer the biologist. Transgenic research will underpin many future applications from evaluating viral vectors for gene therapy and vaccination to bioreactors for the production of proteins, from models of disease to screening tools for biological activity, from genome mapping to gene function. This book details protocols to let you participate in this." -Cloning and Stem Cells
Editors and Affiliations
Bibliographic Information
Book Title: Transgenesis Techniques
Book Subtitle: Principles and Protocols
Editors: Alan R. Clarke
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1592591787
Publisher: Humana Totowa, NJ
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eBook Packages: Springer Book Archive
Copyright Information: Humana Press 2002
eBook ISBN: 978-1-59259-178-7Published: 05 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: X, 351
Topics: Cell Biology