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Telomeres and Telomerase

Methods and Protocols

  • Book
  • © 2002

Overview

Editors:
  1. John A. Double

    1. Cancer Research Unit, University of Bradford Bradford, West Yorkshire, UK

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  2. Michael J. Thompson

    1. Cancer Research Unit, University of Bradford Bradford, West Yorkshire, UK

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 191)

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About this book

The fundamental problem that dividing cells have to ov- come is that of end-replication. Chromosomes shorten by many bases during DNA replication and so this presents a major hurdle that a cell has to overcome both to enable it to proliferate and for the larger organism to survive and reproduce. The enzyme telomerase provides a mechanism to ensure chromosome stability in both normal and neoplastic cells. The demonstration of telomerase expression in a majority of tumors and the realization of the potential role of telomerase in aging has opened up the potential for telomerase to be used as a target for therapeutic intervention. There is therefore great interest in the expression and activity of telomerase in a wide range of biological disciplines. Telomeres and Telomerase: Methods and Protocols has been produced as a tool for the many researchers in different areas of cell biology who are interested in following research in the area of telomerase and telomere maintenance, either in the area of fundamental mec- nisms or perhaps in the area of more applied drug discovery work.

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to Telomeres and Telomerase

    • Michael C. Bibby
    Pages 1-12

  3. Detection of Chromosome Ends by Telomere FISH

    • Harry Scherthan
    Pages 13-31

  4. Telomere Length Distribution

    • Jean-Patrick Pommier, Laure Sabatier
    Pages 33-63

  5. Analysis of Telomerase RNA Gene Expression by In Situ Hybridization

    • W. Nicol Keith, Joseph Sarvesvaran, Martin Downey
    Pages 65-81

  6. Relative Gene Expression in Normal and Tumor Tissue by Quantitative RT-PCR

    • Dennis S. Salonga, Kathleen D. Danenberg, Jean Grem, Ji Min Park, Peter V. Danenberg
    Pages 83-98

  7. Quantitative Detection of Telomerase Components by Real-Time, Online RT-PCR Analysis with the LightCycler

    • Thomas Emrich, Sheng-Yung Chang, Gerlinde Karl, Birgit Panzinger, Chris Santini
    Pages 99-108

  8. Standard TRAP Assay

    • Angelika M. Burger
    Pages 109-124

  9. Stretch PCR Assay

    • Jun-ichi Nakayama, Fuyuki Ishikawa
    Pages 125-136

  10. Fluorescent Detection of Telomerase Activity

    • Wade K. Aldous, Amber J. Marean, Mary J. DeHart, Katherine H. Moore
    Pages 137-146

  11. Nonradioactive Detection of Telomerase Activity Using a PCR-ELISA-Based Telomeric Repeat Amplification Protocol

    • Thomas Emrich, Gerlinde Karl
    Pages 147-158

  12. In Situ TRAP Assay Detection of Telomerase Activity in Cytological Preparations

    • Kazuma Ohyashiki, Junko H. Ohyashiki
    Pages 159-164

  13. Biotinylated Primer for Detecting Telomerase Activity Without Amplification

    • Daekyu Sun
    Pages 165-171

  14. Whole-Cell and Microcell Fusion for the Identification of Natural Regulators of Telomerase

    • Henriette Gourdeau, Marsha D. Speevak, Lucie Jetté, Mario Chevrette
    Pages 173-195

  15. Screening with COMPARE Analysis forTelomerase Inhibitors

    • Imad Naasani, Takao Yamori, Takashi Tsuruo
    Pages 197-207

  16. Telomerase as a Therapeutic Target

    • John A. Double
    Pages 209-216

  17. Back Matter

    Pages 217-221
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Reviews

"Among the many experimental protocols described in this book are methods for visualizing, manipulating, and assaying telomeres and telomerase activity. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to study these entities. The book has a utilitarian look and feel, and while illustrations are not a major feature of the book, the ones that are included are of adequate quality. This book is intended and written as a laboratory guide manual. It would be quite useful for anyone doing bench research on telomeres and telomerase."-Doody's Health Sciences Book Review Journal

Editors and Affiliations

  • Cancer Research Unit, University of Bradford Bradford, West Yorkshire, UK

    John A. Double, Michael J. Thompson

Bibliographic Information

  • Book Title: Telomeres and Telomerase

  • Book Subtitle: Methods and Protocols

  • Editors: John A. Double, Michael J. Thompson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591892

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Humana Press 2002

  • eBook ISBN: 978-1-59259-189-3Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 224

  • Topics: Cell Biology

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