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Cancer Cell Signaling

Methods and Protocols

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Martha Robles-Flores

    1. Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2174)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Cancer Resistance

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Fighting Cancer Resistance: An Overview

      • Martha Robles-Flores
      Pages 3-12

    3. Prostate Cancer Spheroids: A Three-Dimensional Model for Studying Tumor Heterogeneity

      • Mauricio Rodríguez-Dorantes, Carlos David Cruz-Hernandez, Sergio Alberto Cortés-Ramírez, Jenie Marian Cruz-Burgos, Juan Pablo Reyes-Grajeda, Oscar Peralta-Zaragoza et al.
      Pages 13-17

    4. Enrichment and Transcriptional Characterization of Stem Cells Isolated from Human Glioblastoma Cell Lines

      • Ana G. Piña-Medina, Ana M. Hernández-Vega, Néstor F. Díaz, Ismael Mancilla-Herrera, Ignacio Camacho-Arroyo
      Pages 19-29

    5. Reverse Docking for the Identification of Molecular Targets of Anticancer Compounds

      • Angel Jonathan Ruiz-Moreno, Alexander Dömling, Marco Antonio Velasco-Velázquez
      Pages 31-43

    6. Mouse Model for Efficient Simultaneous Targeting of Glycolysis, Glutaminolysis, and De Novo Synthesis of Fatty Acids in Colon Cancer

      • Alejandro Schcolnik-Cabrera, Alfonso Dueñas-Gonzalez
      Pages 45-69

  3. Epigenetic Control of Cancer

    1. Front Matter

      Pages 71-71
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    2. Developing a Portable Device for the Identification of miRNAs in Fluids

      • Alexander Asanov, Alicia Sampieri, Luis Vaca
      Pages 73-88

    3. Methods for the Study of Long Noncoding RNA in Cancer Cell Signaling

      • Yi Feng, Junjie Jiang, Zhongyi Hu, Jiao Yuan, Tianli Zhang, Yutian Pan et al.
      Pages 89-118

    4. RNA-Sequencing Analysis Pipeline for Prognostic Marker Identification in Cancer

      • Sudhanshu Shukla, Seema Khadirnaikar
      Pages 119-131

  4. Metastasis Promotion

    1. Front Matter

      Pages 133-133
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    2. Correlation of Circulating Tumor Cell Measurements with 3D Quantitative Tumor Characterization to Predict Clinical Outcomes in Cancer

      • Sweet Ping Ng, Clifton David Fuller, Heath Devin Skinner
      Pages 135-141

    3. Extracellular Vesicles and Their Roles in Cancer Progression

      • Wen-Hsuan Chang, Richard A. Cerione, Marc A. Antonyak
      Pages 143-170

    4. Analysis of Tumor-Derived Exosomes by Nanoscale Flow Cytometry

      • Cynthia López-Pacheco, Andrea Bedoya-López, Roxana Olguín-Alor, Gloria Soldevila
      Pages 171-191

    5. In Vitro Models for Studying Tumor Progression

      • Juan Carlos González-Orozco, Saúl Gaona-Domínguez, Ignacio Camacho-Arroyo
      Pages 193-206

    6. Assessment of Cell Cycle in Primitive Chronic Myeloid Leukemia Cells by Flow Cytometry After Coculture with Endothelial Cells

      • Patricia Torres-Barrera, Mireya Ramírez-Florencio, Antonieta Chávez-González
      Pages 207-216

  5. New Technologies in the Study of Cancer

    1. Front Matter

      Pages 217-217
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    2. Chimeric Antigen Receptor (CAR) T Cell Therapy for Cancer. Challenges and Opportunities: An Overview

      • Arimelek Cortés-Hernández, Evelyn Katy Alvarez-Salazar, Gloria Soldevila
      Pages 219-244

    3. Cell-Internalization SELEX of RNA Aptamers as a Starting Point for Prostate Cancer Research

      • Mauricio Rodríguez-Dorantes, Sergio Alberto Cortés-Ramírez, Jenie Marian Cruz-Burgos, Juan Pablo Reyes-Grajeda, Alberto Losada-García, Vanessa González-Covarrubias et al.
      Pages 245-254
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Keywords

About this book

This fully updated edition provides the most recent advances in cancer cell signaling knowledge combined with some discussion of the current challenges and prospects in cancer therapy. Beginning with an important section on cancer resistance, the main obstacle to effective cancer therapy, the book continues with chapters exploring state-of-the-art methods in epigenetic control of cancer, metastasis promotion, as well as a variety of new technologies in the study of cancer. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Third Edition serves as an ideal guide to researchers seeking to overcome the challenges in the vital field of cancer cell signaling.

Editors and Affiliations

  • Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico

    Martha Robles-Flores

Bibliographic Information

  • Book Title: Cancer Cell Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Martha Robles-Flores

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0759-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0758-9Published: 20 August 2020

  • Softcover ISBN: 978-1-0716-0761-9Published: 20 August 2021

  • eBook ISBN: 978-1-0716-0759-6Published: 19 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 300

  • Number of Illustrations: 8 b/w illustrations, 47 illustrations in colour

  • Topics: Biomechanics, Cancer Research

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